Asymmetric gramicidin channels: heterodimeric channels with a single F6Val1 residue
نویسندگان
چکیده
منابع مشابه
Gramicidin channels are internally gated.
Gramicidin channels are archetypal molecular subjects for solid-state NMR studies and investigations of single-channel or cation conductance. Until now, the transitions between on and off conductance states have been thought, based on multichannel studies, to represent monomer <--> dimer reactions. Here we use a single-molecule deposition method (vesicle fusion to a planar bilayer) to show that...
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We report here an approach for simultaneous fluorescence imaging and electrical recording of single ion channels in planar bilayer membranes. As a test case, fluorescently labeled (Cy3 and Cy5) gramicidin derivatives were imaged at the single-molecule level using far-field illumination and cooled CCD camera detection. Gramicidin monomers were observed to diffuse in the plane of the membrane wit...
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The interaction of biotin-binding proteins with biotinylated gramicidin (gA5XB) was studied by monitoring single-channel activity and sensitized photoinactivation kinetics. It was discovered that the addition of streptavidin or avidin to the bathing solutions of a bilayer lipid membrane (BLM) with incorporated gA5XB induced the opening of a channel characterized by approximately doubled single-...
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Among all the topics about price dispersion, asymmetric price dispersion between online and o¤-line channels and asymmetric pricing across traditional retailers, e-tailers, and multichannel retailers have received the most extensive empirical examination in the literature. However, in many cases, these studies generate contradictory results. This paper jointly models traditional retailer, e-tai...
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1 This research was conducted in part while Micah Adler was at the Computer Science Department of the University of Toronto (supported by an operating grant from the Natural Sciences and Engineering Research Council of Canada, and by ITRC, an Ontario Centre of Excellence), and in part while he was at the Heinz Nixdorf Institute Graduate College, Paderborn, Germany. Department of Computer Scienc...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1994
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(94)80976-2